Lab Protocols
Protocols for Generating, Manipulating, and Analyzing Pancreatic Organoid Cultures
1. Lab Organoid Protocols (2017 Version)
2. Single Cell dissociation from Organoids
Requests for Reagents
All requests for reagents should be directed to David Tuveson (dtuveson@cshl.edu).
Kras-LSL-G12D and Pdx1-Cre mice can be purchased directly from the Jackson Laboratory:
- Purchasing information for the Kras-LSL-G12D mouse on the B6 background ( B6.129S4-Krastm4Tyj/J): https://www.jax.org/strain/008179
- Purchasing information for the Pdx1-Cre mouse on the B6 background (B6.FVB-Tg(Ipf1-cre)6Tuv/J): https://www.jax.org/strain/014647
Human patient-derived organoid models of cancer are now available directly through ATCC. As a key member of the Human Cancer Models Initiative (HCMI), the Tuveson Laboratory has generated numerous patient-derived organoid models to serve as a community resource. More information on these models can be found at the HCMI Website.